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Data Outputs

Images, Intensity Profiles and Data Grid

• As comet scoring continues, the results are written to the Analysis panel (figure 5)
• Print images and intensity profiles direct from Komet
• Copy images to Windows Clipboard for use in other programs or reports
• Save images to TIFF, BMP and JPEG formats.
• Apply Histogram Mapping and color palettes for visual presentation of Comet Images (figure 6)

Figure 6 - Examples of comet images showing similar levels of damage, but using different visualization. The top image was acquired with a digital color CCD camera from a specimen labelled with SYBR-Green, showing how the cell would look in the microscope eyepiece. The bottom image has been pseudo- colored with a color scale in which blues represent low intensities ranging to green and red for higher values.

Excel Compatible files and Macros

• As each slide or specimen is completed the data is written out to an ASCII data file (to a user-defined or system managed folder) for import into any preferred analysis program
• Komet's unique Excel macros to simplify and streamline data analysis
• Komet's Excel macros allow plotting of single dose Histograms, multi-dose Histograms (3D)and Dose response curves with error bars (Excel 2000)

Komet Databases & Database Viewer

• If the user selects to save the data to a Komet Database, the data and image of every comet scored is transmitted to a Database
• Data output includes details of scoring session – user, time, date, protocol name and all settings
• Database Viewer provides Decoding for blind-scored studies, essential for GLP purposes
• Create and print a Gallery of comets for each specimen or treatment
• Data can be organized by treatmentgroup and statistical summaries produced for significance and dose response analysis
• Locked Database provides security and validation of data
• Create single specimen Histograms, multi-specimen Histograms (3D) and Response curves with error bars
• Database Viewer can be distributed free of charge with the data for review at other sites
• Integration and comparison of data from multiple databases

Figure 7 - The Excel Macros developed by Andor prior to the development of Komet Database Viewer, which are still supplied with the Komet software.

Figure 8a - Komet Database Viewer loads the scored datasets and displays the analysis data and image of each comet scored. This is the basic View/Review tool and you can scroll down the dataset and view the cells in a kind of “movie” by holding down the keyboard scroll down key.

Figure 8b - This dataset is from a GLP study and shows audit trail, decoding records and summary data performed by the study director. Database Viewer lets you create a Gallery of comet images from a Database. This Gallery can be printed or used for review and training.

Figure 9a (left) - 3D Histogram presents study data where a Dose Response was detected. The progression from Negative control (in grey) through the dose groups to the Positive control (orange) is apparent in the data distributions for Tail DNA. Figure 9b (right) - The data can also be presented in Response Curve format & Summary data table.