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Andor iQ Software

iQ version 1.9 - Release Notes

Andor iQ is our flagship live cell imaging software, designed with flexibility and power in mind. iQ - image and quantify - occupies a central role in our Revolution product range and provides optimized control of Andor's award winning iXon^EM+ EMCCD cameras and automation hardware for a range of Bioimaging applications. Continuous development and improvement ensures that Andor iQ represents a powerful and flexible core for live-cell imaging systems.

Optimized Imaging Software & Choice

• Dell Precision workstation
• Acquisition Bandwidth and System Configuration
• ImageDisk removes dependency on RAM for Huge Datasets
• Multi-Core Processors and Multi-threaded software
• High performance graphics with OpenGL
• High resolution, high dynamic range, fast response LCD Monitors
• PCI cards included in system

The best live cell imaging software just got better - Version 1.9

• FRAPPA – software support was improved in version 1.8.1 and first made available in version 1.8. Once again we have improved its functionality by enabling multi-region bleaching both live and within a protocol. Multi-region FRAPPA is a right mouse menu item on the Image Window and is toggled on/off. When selected all regions will be bleached or activated, when off only the selected region will be bleached. To enable the mode of baseline collection followed by FRAPPA action and then recovery (classic FRAP) the user must Select a FRAPPA region. When no region is Selected the imaging Protocol continues uninterrupted. So the user can now decide at which times FRAPPA action should be initiated and whether all or just one region is bleached or activated.
• FRAPPA is now available in Fast Protocols – but mouse down does block the sequence. iQ 2 will extend multithreading to avoid this issue.
• FRAPPA has been made independent of the PCU-100 series controller and now provides its own Blanking signal to the ALC Laser combiner.
• FRAPPA Calibration Wizard now stores multiple calibration files for different hardware configurations.
• Andor Revolution CSU-X1, FRAPPA, TIRFM solution is an example of a system requiring distinct FRAPPA Calibrations. Built on an IX81 microscope, our optical solution allows FRAPPA to be used with either CSUX or TIRFM. The CSUX detector is used on the left port, while the TIRFM detector is mounted on the right hand port. With a custom dichroic for the right port mirror FRAPPA can be used in combination with TIRFM illumination and detection. However, because the detection paths are dissimilar the FRAPPA angle to pixel mapping (Calibration) each must be stored in its own file. The ImageList sorting bug has been fixed. You can now sort correctly by date, time, name etc.
• A new Filter PlugIn is released with iQ 1.9 providing a range of 3x3 convolution and ranking filters. These included high and low pass and morphological filters - close (minimum), dilate (maximum) and noise removal (median)
• Multi-Field ZDC and PFS capability has been added to the Scan control. One offset value is used for all fields.
• ALC/AOTF controller has been enhanced to allow 0.1% laser power control and capacity for 5 solid state laser lines – see the ALC-501 series. Support for a new 594 nm laser line is now included.
• CoolLED PrecisExcite LED source is now available globally from Andor and its distributors. It integrated into iQ and is available in a bundle configuration with Luca-S or Luca-R and workstation.
• Device Setup – the Record function has been simplified by removing the drop-down list and providing a separate New button. On clicking Record the current channel settings are updated with those in the current device control panels.
• CSU-X1 filter wheel speed control has been added to allow vibration reduction in sensitive imaging experiments.